RESTRICTION FRAGMENT LENGTH POLYMORPHISM

'\Restriction fragment length polymorphism' (or RFLP) is a property of DNA used in molecular biology methods, for example, genetic fingerprinting or paternity testing.

Method

Usually, DNA from an individual organism is first amplified (multiple copies are made) by the polymerase chain reaction (PCR). It is then cut into restriction fragments at defined positions by restriction enzymes. The restriction fragments are then separated according to length by agarose gel electrophoresis. The resulting gel may be enhanced by Southern blotting.

Result

The distance between the locations cut by restriction enzymes (the restriction sites) varies between individuals: so the length of the fragments varies, and the position of certain gel bands differs between individuals (thus polymorphism). This can be used to genetically tell individuals apart. It can also show the genetic relationship between individuals, because children inherit genetic elements from their parents. It is also used to deterimne the relationships among species.

See also: genetics -- retroposon

Commercial Usage: RESTRICTION FRAGMENT LENGTH POLYMORPHISM

Frequency of Internet Keywords: RESTRICTION FRAGMENT LENGTH POLYMORPHISM

Modern Translation: RESTRICTION FRAGMENT LENGTH POLYMORPHISM

Language		Translations for "RESTRICTION FRAGMENT LENGTH POLYMORPHISM"; alternative meanings/domain in parentheses.
Danish		RFLP-kortlægning (RFLP mapping). (various references)

Dutch		Restriction Fragment Length Polymorphism, restrictiefragmentlengte-polymorfisme. (various references)

Finnish		DNA:n katkoskirjo. (various references)

French		polymorphisme des fragments de restriction, polymorphisme de taille des fragments de restriction (restricted fragment length polymorphism), polymorphisme de restriction du DNA, polymorphisme de restriction. (various references)

German		Restriktionspolymorphismus, Restriktionsfragmentlängen-Polymorphismus. (various references)

Greek		πολυμορφισμός μυικών θραυσμάτων λαμβανομένων με περιοριστικά ένζυμα(RFLP), πολυμορφισμός μεγέθους περιοριστικών θραυσμάτων, πολυμορφισμός περιορισμού του DNA. (various references)

Italian		restriction fragment length polymorphism, polimorfismo di lunghezza del frammento di restrizione, polimorfismo della lunghezza del frammento di restrizione, polimorfismi di siti restrizione, polimorfismi di restrizione del DNA. (various references)

Pig Latin		estrictionray agmentfray engthlay olymorphismpay

Spanish		polimorfismo restrictivo de fragmento largo, polimorfismo por restricción de longitud de fragmentos, polimorfismo en los tamaños de los fragmentos de restricción, polimorfismo de longitud de fragmentos de restricción. (various references)
Source: compiled by the editor from various translation references.

Alternative Orthography: RESTRICTION FRAGMENT LENGTH POLYMORPHISM

Hexadecimal (or equivalents, 770AD-1900s) (references)

52 45 53 54 52 49 43 54 49 4F 4E 46 52 41 47 4D 45 4E 54 4C 45 4E 47 54 48 50 4F 4C 59 4D 4F 52 50 48 49 53 4D

Leonardo da Vinci (1452-1519; backwards) (references)

Binary Code (1918-1938, probably earlier) (references)

01010010 01000101 01010011 01010100 01010010 01001001 01000011 01010100 01001001 01001111 01001110 00100000 01000110 01010010 01000001 01000111 01001101 01000101 01001110 01010100 00100000 01001100 01000101 01001110 01000111 01010100 01001000 00100000 01010000 01001111 01001100 01011001 01001101 01001111 01010010 01010000 01001000 01001001 01010011 01001101

HTML Code (1990) (references)

&#82 &#69 &#83 &#84 &#82 &#73 &#67 &#84 &#73 &#79 &#78 &#32 &#70 &#82 &#65 &#71 &#77 &#69 &#78 &#84 &#32 &#76 &#69 &#78 &#71 &#84 &#72 &#32 &#80 &#79 &#76 &#89 &#77 &#79 &#82 &#80 &#72 &#73 &#83 &#77

ISO 10646 (1991-1993) (references)

0052 0045 0053 0054 0052 0049 0043 0054 0049 004F 004E 0046 0052 0041 0047 004D 0045 004E 0054 004C 0045 004E 0047 0054 0048 0050 004F 004C 0059 004D 004F 0052 0050 0048 0049 0053 004D

Encryption (beginner's substitution cypher): (references)

Domain	Definition
Medicine	Variation in the length of restriction fragments that are generated after DNA is digested with different restriction enzymes and analysed with a variety of cloned DNA probes which detect specific homologous DNA fragments. Source: European Union. (references)
Source: compiled by the editor from various references; see credits.

Domain	Title
Books	Restriction Fragment Length Polymorphism (reference) (more book examples)
Source: compiled by the editor from various references; see credits.

Expression	Frequency per Day
restriction fragment length polymorphism	6
Source: compiled by the editor from various references; see credits.

RESTRICTION FRAGMENT LENGTH POLYMORPHISM

Specialty Definition: RESTRICTION FRAGMENT LENGTH POLYMORPHISM

Medicine

Specialty Definition: Restriction fragment length polymorphism

Method

Result

Crosswords: RESTRICTION FRAGMENT LENGTH POLYMORPHISM

Commercial Usage: RESTRICTION FRAGMENT LENGTH POLYMORPHISM

Books

Frequency of Internet Keywords: RESTRICTION FRAGMENT LENGTH POLYMORPHISM

restriction fragment length polymorphism

Modern Translation: RESTRICTION FRAGMENT LENGTH POLYMORPHISM

Danish

Dutch

Finnish

French

German

Greek

Italian

Pig Latin

Spanish

Alternative Orthography: RESTRICTION FRAGMENT LENGTH POLYMORPHISM

52 45 53 54 52 49 43 54 49 4F 4E 46 52 41 47 4D 45 4E 54 4C 45 4E 47 54 48 50 4F 4C 59 4D 4F 52 50 48 49 53 4D

&#82 &#69 &#83 &#84 &#82 &#73 &#67 &#84 &#73 &#79 &#78 &#32 &#70 &#82 &#65 &#71 &#77 &#69 &#78 &#84 &#32 &#76 &#69 &#78 &#71 &#84 &#72 &#32 &#80 &#79 &#76 &#89 &#77 &#79 &#82 &#80 &#72 &#73 &#83 &#77

0052 0045 0053 0054 0052 0049 0043 0054 0049 004F 004E 0046 0052 0041 0047 004D 0045 004E 0054 004C 0045 004E 0047 0054 0048 0050 004F 004C 0059 004D 004F 0052 0050 0048 0049 0053 004D

52395354524337544349482405235414739485424639484154422504946594749525042435347

INDEX