De novo cell-free synthesis of picornavirus

Year	Description
1994	Invention patented by Eckard Wimmer, Akhteruzzaman Molla, and Aniko V. Paul on June 29th, 1994. Abstract: A process for the de novo synthesis of a picornavirus using a cell-free medium has been developed. The cell-free medium is prepared from a lysate of mammalian cells from which the nuclei and mitochondria has been removed, endogenous mRNA deactivated and supplemented with nucleoside triphosphates, tRNA, amino acids and precursors necessary for generating proteins. The genomic RNA of the picornavirus or rhinovirus is added to the cell-free medium and after incubation at about 30.degree. C. to 40.degree. C. of from about 4 to 24 hours infectious, mature virus particles are formed.
Source: selected by the editor from original sources.

Invention: De novo cell-free synthesis of picornavirus