DNA ELECTROPHORESIS

  

Copyright © Philip M. Parker, INSEAD. Terms of Use.

DNA ELECTROPHORESIS

Specialty Definition: DNA electrophoresis

(From Wikipedia, the free Encyclopedia)

DNA electrophoresis is an analytical technique used to separate DNA fragments by dimension. It is an example of chromatography. Basically, an electric field forces the fragments to migrate in a gel. DNA molecules normally migrate from negative to positive pole due to the net negative charge of the phosphate backbone of the DNA chain. The molecules are forced to pass through the gel, that at molecular length scale looks much like a random, intricated network. Long molecules will migrate slower, because it is easier for them to be 'trapped' in the network, and short molecules will migrate faster. Once the migration is completed, the fractions of DNA fragments of different length can be seen using a fluorescent dye specific for DNA, like ethidium bromide. The gel will show little,more or less thin bands corresponding to different DNA molecules populations with different molecular weight.

The main DNA electrophoresis technique is Agarose gel electrophoresis. Other techniques used are Polyacrylamide gel electrophoresis, a technique used for proteins but that's handy also for short DNA molecules,and capillary electrophoresis.

Source: the above text is adapted by the editor from Wikipedia, the free encyclopedia under a copyleft GNU Free Documentation License (GFDL) from the article "DNA electrophoresis."

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Copyright © Philip M. Parker, INSEAD. Terms of Use.